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DNA lab: useful links

This is a list of additional resources for the DNA lab theme and information sheets.

DNA extraction

DNA extraction animation
An interactive showing the steps of DNA extraction, from Learn.Genetics – an online Genetics Science Learning Centre run by the University of Utah.
http://learn.genetics.utah.edu/content/labs/extraction/

Restriction enzymes

Examples of restriction enzymes and their recognition sites
Information about restriction enzymes created specifically for high schools by the University of Otago.
highschoolbiology.otago.ac.nz...option=com_content&view=article&id=112&Itemid=96

A closer look at restriction enzymes
A detailed explanation of restriction enzyme activity, including specific examples, developed by Colorado State University.
www.vivo.colostate.edu/hbooks/genetics/biotech/enzymes/index.html

DNA ligation

A closer look at ligation
A detailed explanation of ligation, including scientists proving that DNA fragments have been joined together by ligation. Developed by Colorado State University.
www.vivo.colostate.edu/hbooks/genetics/biotech/enzymes/ligation.html

Bacterial transformation

Bacterial cell structure
Get to know more about what’s inside a bacterial cell, from CELLS alive!
www.cellsalive.com/cells/bactcell.htm

DNA, plasmids and bacterial transformation
Examples of plasmids that are used in the laboratory for bacterial transformation, from Dr John Kimball at Harvard University.
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html

Bacterial transformation
A step-by-step guide to doing a bacterial transformation in the lab, from Plattsburgh State University of New York.
http://faculty.plattsburgh.edu/donald.slish/Transformation.html

Making recombinant DNA in the classroom
A simple cut and paste paper activity to demonstrate DNA cloning, created by the Health and Bioscience Teaching Resource Centre of the National Museum in Washington.
www.accessexcellence.org/RC/AB/WYW/wkbooks/SFTS/activity6.html

Polymerase chain reaction (PCR)

Polymerase chain reaction animation
An interactive showing the steps of PCR, from Learn.Genetics – an online Genetics Science Learning Centre run by the University of Utah.
http://learn.genetics.utah.edu/content/labs/pcr

Gel electrophoresis

Gel electrophoresis (Colorado State University Biotechnology Index)
This site provides an excellent explanation of gel electrophoresis and has a link to a very good interactive activity for senior biology students.
http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/agardna.html

DNA sequencing

The University of Michigan DNA Sequencing Core
An excellent explanation of the Sanger (di-deoxy) DNA sequencing method, with very clear diagrams.
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html

Using maths to make sense of genetic data.
In this episode of Ever Wondered? Series 2, on the Science Learning Hub, Dr John Watt talks to Professor Mike Steel who is finding ways to use maths to make sense of all the questions provided by the abundance of genetic data produced by modern sequencing techniques.
www.sciencelearn.org.nz/Science-Stories/Ever-Wondered-Series-2/Maths

Microarrays/DNA chips

Anatomy of a comparative gene expression study
Detailed explanations about how microarrays are made and used, in very user-friendly language.
www.cs.wustl.edu/~jbuhler/research/array/

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